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Heteronomous DNA.

S Arnott, R Chandrasekaran, I H Hall

    Nucleic Acids Research
    |June 25, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Researchers discovered a novel heteronomous secondary structure in fibrous polydeoxyadenylic:deoxythymidylic acid (poly d(A):poly d(T)). This finding reveals distinct conformations within the same polynucleotide duplex, expanding our understanding of DNA structures.

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    Area of Science:

    • Molecular Biology
    • Biochemistry
    • Structural Biology

    Background:

    • Polynucleotide duplexes typically exhibit uniform secondary structures.
    • The conformational diversity of nucleic acid double helices is crucial for their function.
    • Previous studies have not confirmed heteronomous structures in polynucleotide duplexes.

    Purpose of the Study:

    • To investigate the secondary structure of fibrous polydeoxyadenylic:deoxythymidylic acid (poly d(A):poly d(T)).
    • To determine if polynucleotide duplexes can possess distinct conformations within the same double helix.
    • To explore the implications of heteronomous structures for nucleic acid polymorphism.

    Main Methods:

    • X-ray diffraction analysis of fibrous poly d(A):poly d(T).
    • Conformational analysis of individual polynucleotide chains.

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  • Comparison of observed structures with known nucleic acid conformations (A-form and B-form).

    • Main Results:

    • A fibrous form of poly d(A):poly d(T) exhibits a confirmed heteronomous secondary structure.
    • Both polynucleotide chains adopt 10(1) helical conformations, hydrogen-bonded in the Watson-Crick fashion.
    • One chain (likely poly d(A)) displays C3'-endo puckering (A-family), while the other (likely poly d(T)) shows C2'-endo puckering (B-family).

    Conclusions:

    • This is the first confirmed instance of a heteronomous secondary structure in a polynucleotide duplex.
    • Analogous heteronomous structures may exist in DNA-DNA and DNA-RNA duplexes with varied base sequences.
    • The polymorphic range of polynucleotide double helices might be more extensive than previously recognized.