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Albuminated polymer surfaces for biomedical application.

R Sipehia, A S Chawla

    Biomaterials, Medical Devices, and Artificial Organs
    |January 1, 1983
    PubMed
    Summary
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    Amino groups were successfully grafted onto Celgard-2400 membranes using ammonia plasma, enabling albumin attachment. The modified membranes demonstrated stable albumin retention after washing, indicating potential for biomaterial applications.

    Area of Science:

    • Materials Science
    • Surface Chemistry
    • Biomaterials Engineering

    Background:

    • Celgard-2400 membranes are widely used but require surface modification for specific biological applications.
    • Protein immobilization is crucial for developing advanced biomaterials and biosensors.
    • Plasma treatment offers a versatile method for surface functionalization.

    Purpose of the Study:

    • To functionalize Celgard-2400 membranes with amino groups using ammonia plasma.
    • To immobilize albumin onto the amino-functionalized membranes.
    • To assess the stability and retention of immobilized albumin.

    Main Methods:

    • Surface modification of Celgard-2400 membranes via ammonia plasma treatment.
    • Characterization of amino group presence using Attenuated Total Reflection Fourier Transform Infrared Spectroscopy (ATR-FTIR) and Auger Electron Spectroscopy (AES).

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  • Albumin attachment and stabilization via glutaraldehyde cross-linking, followed by washing experiments.

    • Main Results:

    • Successful introduction of amino groups onto the membrane surface was confirmed.
    • Albumin was effectively attached to the functionalized membranes.
    • Washing experiments showed a leveling-off concentration of retained albumin, with amounts ranging from 275 to 357 µg/cm².

    Conclusions:

    • Ammonia plasma treatment is an effective method for amino-functionalizing Celgard-2400 membranes.
    • The functionalized membranes can stably immobilize albumin, with potential for controlled protein delivery or surface bio-functionalization.
    • The results suggest the developed method is suitable for creating robust protein-coated membranes.