Chromatographic separation of oligosaccharides from mannosidosis urine

A mixture of oligosaccharides was isolated from mannosidosis urine by a rapid and convenient procedure employing adsorption on activated charcoal. The mixture was partially fractionated into a homologous series of compounds by a rapid procedure employing a preparative, liquid chromatograph, and a more complete separation was obtained by a second chromatographic step employing a solid phase having more-powerful resolving properties, or by preparative-layer chromatography. The series of oligosaccharides was completely separated by 7-MPa, liquid chromatography (l.c.) on a Micropak NH2-10 column; the analysis could be performed with isocratic or gradient solvent-systems, and did not involve derivatization. With the isocratic system, a strict relationship was observed between retention time and the number of D-mannosyl residues. The use of 1,4-diaminobutane as a column "restorer" was evaluated.