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Simple procedure for the isolation of mouse interferon-beta
Abstract:
We report a simple procedure for the isolation of a mouse interferon beta (beta IFN) from Ehrlich ascites tumor cells infected with Newcastle disease virus. The procedure consists of four chromatographic steps (two on controlled pore glass, one on CM-Sephadex, and one on phosphocellulose) with stepwise elution. The product purified is a 35-40 kD beta IFN with a specific activity of 10(9) units/mg protein.