Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Large agarose beads for extracorporeal detoxification systems.

H Lösgen, G Brunner, C J Holloway

    Biomaterials, Medical Devices, and Artificial Organs
    |January 1, 1978
    PubMed
    Summary

    Researchers developed large agarose beads for hemoperfusion, showing good hemocompatibility and potential for extracorporeal detoxification in liver failure.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Myocardial fibrosis occurs in non-hospitalised patients with chronic symptoms after COVID-19.

    International journal of cardiology. Heart & vasculature·2022
    Same author

    Antitumor effects and hematotoxicity of C<sub>60</sub>-Cis-Pt nanocomplex in mice with Lewis lung carcinoma.

    Experimental oncology·2019
    Same author

    Correction: The Relative Contribution of Metabolic and Structural Abnormalities to Diastolic Dysfunction in Obesity.

    International journal of obesity (2005)·2019
    Same author

    The relative contribution of metabolic and structural abnormalities to diastolic dysfunction in obesity.

    International journal of obesity (2005)·2017
    Same author

    C<sub>60</sub> Fullerene as Promising Therapeutic Agent for the Prevention and Correction of Skeletal Muscle Functioning at Ischemic Injury.

    Nanoscale research letters·2017
    Same author

    Lower coronary plaque burden in patients with HIV presenting with acute coronary syndrome.

    Open heart·2017

    Area of Science:

    • Biomaterials Science
    • Medical Engineering
    • Biochemistry

    Background:

    • Traditional hemoperfusion materials often cause significant platelet loss and hemolysis.
    • Existing agarose beads (Sepharose 4B) have small diameters (40-190 microns).
    • Need for improved carrier matrices for extracorporeal detoxification and artificial support systems.

    Purpose of the Study:

    • To develop a method for preparing larger agarose beads (1000-10000 microns) from Sepharose 4B.
    • To evaluate the hemocompatibility of these enlarged beads.
    • To assess their suitability for protein immobilization and potential medical applications like extracorporeal detoxification.

    Main Methods:

    • Preparation of enlarged agarose beads from Sepharose 4B.
    • Hemoperfusion experiments to assess hemocompatibility (platelet loss, hemolysis).

    Related Experiment Videos

  • Activation of beads using cyanogen bromide for protein immobilization.
  • Diffusion studies for small molecules and proteins within the beads.

    • Main Results:

    • Successfully prepared agarose beads with diameters ranging from 1000 to 10000 microns.
    • Enlarged beads demonstrated relative hemocompatibility with significantly reduced platelet loss and slight hemolysis.
    • Protein immobilization is feasible, with accessible sites throughout the bead matrix.
    • Rapid diffusion of small molecules and proteins within the enlarged beads was observed.

    Conclusions:

    • The developed method yields large, hemocompatible agarose beads suitable for medical applications.
    • These beads can serve as effective carrier matrices for extracorporeal detoxification, particularly for lipophilic toxins.
    • The enlarged beads show promise as artificial supports in treating conditions like fulminant hepatic failure.