A procedure for obtaining initial estimates of parameters appearing in steady-state rate or equilibrium binding...

A "peeling" procedure is described for obtaining initial estimates of the parameters in the equation: formula: (see text), where P(x) and Q(x) are polynomials in x. The method is illustrated, in the context of enzyme kinetics, using data which are fitted to the following equation: formula: (see text), where v denotes the initial steady-state velocity at an initial substrate concentration S, and a1, a2, b1, and b2 are non-negative constants. The applicability and limitations of the method for data fitting in fields such as enzyme kinetics and ligand-binding studies are discussed.