Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Phospholipid methylation in myogenic cells.

T K Koch, A S Gordon, I Diamond

    Biochemical and Biophysical Research Communications
    |July 18, 1983
    PubMed
    Summary

    Phosphatidylcholine biosynthesis via phosphatidylethanolamine methylation is key for cell signaling. While L-isoproterenol stimulated adenylate cyclase in myogenic cells, the phospholipid methylation pathway remained unchanged.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Genetic variation among 82 pharmacogenes: The PGRNseq data from the eMERGE network.

    Clinical pharmacology and therapeutics·2016
    Same author

    Design and anticipated outcomes of the eMERGE-PGx project: a multicenter pilot for preemptive pharmacogenomics in electronic health record systems.

    Clinical pharmacology and therapeutics·2014
    Same author

    Manual artificial respiration.

    What's new·2014
    Same author

    Adsorption mediated decrease in the biodegradation rate of organic compounds.

    Microbial ecology·2013
    Same author

    The adrenal gland and phagocytosis in the spleen.

    Federation proceedings·2010
    Same author

    Antihormone reactions to blood, urinary and pituitary gonadotrophins.

    Federation proceedings·2010

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Molecular Signaling

    Background:

    • Phosphatidylcholine biosynthesis from phosphatidylethanolamine involves N-methylation.
    • This pathway is crucial for signal transduction, including beta-adrenergic receptor coupling to adenylate cyclase.

    Purpose of the Study:

    • To investigate the role of membrane phospholipid methylation in beta-adrenergic signal transduction.
    • To examine L-isoproterenol's effect on adenylate cyclase activity and phospholipid methylation in myogenic cell lines.

    Main Methods:

    • Utilized two myogenic cell lines (L8 and BC3H-1).
    • Stimulated cells with L-isoproterenol to measure adenylate cyclase activity.
    • Employed high-performance liquid chromatography (HPLC) for phospholipid analysis.

    Main Results:

    • Demonstrated L-isoproterenol stimulation of adenylate cyclase activity in both cell lines.
    • Confirmed the presence of an active membrane phospholipid methylation pathway.
    • Observed no significant alteration in the transmethylation pathway despite beta-adrenergic stimulation.

    Conclusions:

    • The N-methylation pathway of phosphatidylcholine biosynthesis is active in L8 and BC3H-1 cells.
    • Beta-adrenergic stimulation of adenylate cyclase does not appear to directly modulate this specific phospholipid transmethylation pathway.

    Related Experiment Videos