[Chronology of the appearance of embryonic ossification nuclei in the turkey]
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Updated: Aug 17, 2026

Optimized Ex-ovo Culturing of Chick Embryos to Advanced Stages of Development
Published on: January 24, 2015
Abstract:
252 turkey eggs were put in an incubator at 39 degrees C and relative humidity 75%. From the 8th day of incubation up to the 28th, the authors opened 12 eggs a day, 6 at 8 a.m. and 6 at 8 p.m. The embryos were fixed by 10% buffered formaline for three days, dehydrated by alcohol 96% for seven days, eviscerated, coloured by alizarin S red and by alcian blue 8GX, then made diaphanans by means of KOH solutions. Then the authors have described the appearance times of the ossification nuclei in turkey, showing that in turkey the embryonal ossification occurs in a characteristic chronological order, different from that described in other birds, even if they belong to related species.
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