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Hemopoietic stem cells in the liver of mice with Friend leukemia
Abstract:
Hemopoietic stem cells were studied in the marrow, spleen and liver of individual, FV-P infected DBA/2 mice. At more than 17 days after infection there was a tendency for CFU-EI numbers in the spleen to be reduced compared to earlier intervals after virus infection; CFU-EI numbers in the marrow were low. At the same time all hemopoietic stem cells (CFU-S, CFU-C, BFU-E and CFU-EI) could be found in the enlarged liver. The migration of stem cells from the marrow to the spleen and further to the liver is discussed.
Insights
Following FV-P virus infection, hemopoietic stem cell (HSC) numbers decrease in mouse spleen and marrow. All HSC types migrate to the enlarged liver, indicating a significant shift in stem cell localization.
Area of Science:
- Hematology
- Virology
- Immunology
Background:
- FV-P virus infection impacts DBA/2 mice, affecting hemopoietic stem cell (HSC) populations.
- Understanding stem cell dynamics is crucial for comprehending host responses to viral infections.
Purpose of the Study:
- To investigate the distribution and changes in various hemopoietic stem cell populations (CFU-S, CFU-C, BFU-E, CFU-EI) in DBA/2 mice post-FV-P infection.
- To determine the migration patterns of stem cells from marrow to spleen and liver.
Main Methods:
- Studying hemopoietic stem cells in the marrow, spleen, and liver of individual FV-P infected DBA/2 mice.
- Quantifying colony-forming units (CFU) for different stem cell types at various time points post-infection.
Main Results:
- A reduction in CFU-El numbers was observed in the spleen after 17 days of infection.
- Low CFU-EI numbers were detected in the bone marrow.
- All studied hemopoietic stem cell types were found in the enlarged liver.
Conclusions:
- FV-P infection induces a migration of hemopoietic stem cells from the marrow and spleen to the liver.
- The liver becomes a significant site for hemopoietic stem cells during later stages of FV-P infection.