The ultrastructure of rat rod synaptic terminals: effects of dark-adaptation

The ultrastructure of synaptic terminals of rat rod photoreceptors was studied in light-adapted and dark-adapted states. In the light-adapted state, horizontal cell processes embedded in the photoreceptor terminal show smooth surface contours and are ovoid in cross section. In the dark-adapted state, fingerlike protrusions of photoreceptor cytoplasm extend into the horizontal cell processes on either side of the synaptic ribbon. An electron-dense structure lies under the horizontal cell membrane that surrounds these fingerlike protrusions. This submembrane specialization is generally associated with areas of concavity of the horizontal cell membrane; it occurs in goldfish rods as well as rat rods. The protrusions and the horizontal cell specialization may be involved in membrane dynamics during synaptic activity.