Direct solid-phase 125I radioimmunoassay of serum cortisol

We report a solid-phase iodine- 125 radioimmunoassay for serum cortisol in which the interfering binding proteins are inactivated by a combination of reaction pH and 8-anilino-1-naphthalenesulfonic acid. The procedure is easy to perform and gives accurate and reliable results. The shift in pH for optimum binding of an antibody resulting from immobilization on a solid support, used to decrease cortisol binding competition in the present test, is potentially exploitable in other antibody and enzyme systems.