Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Variable region (Fv) determinants on mouse lymphocytes.

I McConnell, P J Lachmann, D Givol

    Immunology
    |June 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    One Health in the context of medical and veterinary education.

    Revue scientifique et technique (International Office of Epizootics)·2015
    Same author

    Regulation of p53 activity by HIPK2: molecular mechanisms and therapeutical implications in human cancer cells.

    Oncogene·2010
    Same author

    E-cadherin expression in canine cutaneous histiocytomas.

    The Veterinary record·2008
    Same author

    Cloning and sequencing of ovine Flt3 ligand.

    International journal of immunogenetics·2007
    Same author

    Gene expression profiles of AML derived stem cells; similarity to hematopoietic stem cells.

    Leukemia·2006
    Same author

    S100A2 gene is a direct transcriptional target of p53 homologues during keratinocyte differentiation.

    Oncogene·2006

    Mouse lymphocytes possess Fv fragment determinants, primarily on B cells, which are part of the B-cell antigen receptor. T cells, including T-helper cells, do not express these immunoglobulin v region framework determinants.

    Area of Science:

    • Immunology
    • Cell Biology
    • Molecular Biology

    Background:

    • Antisera were generated against the Fv fragment of a mouse myeloma protein.
    • These antisera exhibited varied reactivity with Fv fragments and mouse immunoglobulins.
    • Two antisera identified framework determinants within the variable (v) region of normal mouse immunoglobulins.

    Purpose of the Study:

    • To investigate the presence and distribution of Fv fragment determinants on mouse lymphocytes.
    • To determine if these determinants are expressed on B lymphocytes and T lymphocytes.
    • To ascertain if T cells express immunoglobulin v region framework determinants.

    Main Methods:

    • Generation of antisera against mouse myeloma Fv fragment.
    • Immunological assays to test reactivity with Fv, immunoglobulins, and lymphocyte surface determinants.

    Related Experiment Videos

  • Flow cytometry or similar techniques to analyze lymphocyte populations.

    • Main Results:

    • A significant population of mouse lymphocytes displayed Fv determinants on their surface.
    • These determinants were identified as part of the B-lymphocyte antigen receptor.
    • The majority of lymphocytes expressing Fv determinants were B lymphocytes.
    • Fv determinants were largely absent from T lymphocytes, including T-helper cells.

    Conclusions:

    • In mice, B lymphocytes express Fv fragment determinants as part of their antigen receptor.
    • T lymphocytes, including T-helper cells, do not express immunoglobulin v region framework determinants on their cell surface.