GLC determination of alpha- and beta-tribenosides in human plasma

The determination of alpha-tribenoside at concentrations down to 10 ng/ml and beta-tribenoside at concentrations down to 5 ng/ml in human plasma is described. After addition of an internal standard, alpha- and beta-tribenosides are extracted at basic pH into benzene. Both compounds are derivatized with N-heptafluorobutyrylimidazole. The derivatives are determined by GLC using a 63Ni-electron-capture detector.