9-(5-Carboxypentylamino)-acridine. An affinity adsorbent ligand for Electrophorus acetylcholinesterase

We have developed a simple method for the synthesis of the ligand 9-(5-carboxypentylamino)-acridine and the resulting affinity adsorbent using Sepharose CL-4B. This affinity adsorbent is efficient and specific for purification acetylcholinesterase (acetylcholine acetylhydrolase, EC 3.1.1.7) from Electrophorus electricus electric organ.