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A modified allochrome procedure for demonstrating mycobacteria in tissue sections.

K Harada

    International Journal of Leprosy and Other Mycobacterial Diseases : Official Organ of the International Leprosy Association
    |January 1, 1977
    PubMed
    Summary

    A new allochrome staining method reliably detects mycobacteria in tissue. This sensitive technique stains the bacteria bright red against the background, revealing otherwise invisible bacilli.

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    Area of Science:

    • Histopathology
    • Microbiology
    • Staining Techniques

    Background:

    • Accurate identification of mycobacteria in tissue is crucial for diagnosing infections.
    • Existing staining methods may lack sensitivity or reliability for certain mycobacteria.
    • The need for improved diagnostic tools in mycobacterial research is ongoing.

    Purpose of the Study:

    • To present a modified allochrome staining procedure for enhanced demonstration of mycobacteria.
    • To establish a reliable and sensitive method for visualizing mycobacteria in tissue sections.
    • To improve the detection of otherwise chromophobic mycobacteria.

    Main Methods:

    • A modified allochrome staining technique was developed.
    • The procedure involves deparaffinization, oxidation with periodic acid, differentiation with HCl-ethanol, nuclear staining with Weigert's iron hematoxylin, and counterstaining with picro-methyl glue.

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  • Formalin-fixed tissue sections were utilized.

    • Main Results:

    • The modified allochrome staining procedure demonstrated high reliability and sensitivity.
    • Mycobacteria were stained a brilliant red, providing clear contrast against the background.
    • The method successfully visualized bacilli that were otherwise chromophobic.

    Conclusions:

    • The modified allochrome staining procedure is a superior method for demonstrating mycobacteria in tissue sections.
    • This technique offers improved sensitivity and reliability for diagnosing mycobacterial infections.
    • The bright red staining facilitates the identification of even difficult-to-detect mycobacteria.