Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lateral diffusion in inhomogeneous membranes. Model membranes containing cholesterol.

J C Owicki, H M McConnell

    Biophysical Journal
    |June 1, 1980
    PubMed
    Summary

    Inhomogeneous membranes show complex lipid diffusion. Theoretical calculations reveal how small-scale membrane domains influence large-scale diffusion measurements, matching experimental findings.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Baseline hydrocarbon levels in New Zealand coastal and marine avifauna.

    Marine pollution bulletin·2015
    Same author

    Testing ocular irritancy in vitro with the silicon microphysiometer.

    Toxicology in vitro : an international journal published in association with BIBRA·2010
    Same author

    Spin-labeled membranes.

    The Journal of general physiology·2009
    Same author

    Contrast inversion in the epifluorescence of cholesterol-phospholipid monolayers.

    Biophysical journal·2004
    Same author

    Condensed complexes and the calorimetry of cholesterol-phospholipid bilayers.

    Biophysical journal·2001
    Same author

    Kinetics of registry selection of chimeric peptides binding to MHC II.

    Biochemistry·2001

    Area of Science:

    • Membrane biophysics
    • Physical chemistry
    • Computational biology

    Background:

    • Lateral diffusion is crucial for membrane function.
    • Inhomogeneous lipid membranes present challenges for studying diffusion.
    • Fluorescence photobleaching experiments measure diffusion over macroscopic distances.

    Purpose of the Study:

    • To theoretically calculate lateral diffusion of a fluorescent lipid probe in inhomogeneous lipid mixtures.
    • To illustrate how small-scale membrane heterogeneities affect diffusion measurements.
    • To explain the observed dependence of diffusion coefficients on lipid composition.

    Main Methods:

    • Theoretical modeling of lateral diffusion.
    • Simulation of fluorescent lipid probe behavior in binary phosphatidylcholine-cholesterol mixtures.

    Related Experiment Videos

  • Analysis of diffusion in systems with alternating fluid and solid lipid domains.

    • Main Results:

    • Inhomogeneities on a 10-100 nm scale lead to complex diffusive responses at 10-100 micrometer scales.
    • Theoretical calculations reproduce the unusual dependence of apparent lateral diffusion coefficients on lipid composition.
    • The model explains experimental observations by Rubenstein et al. (1979).

    Conclusions:

    • Membrane inhomogeneities significantly impact the measurement of lateral diffusion.
    • Small-scale domain structures can create apparent diffusion complexities in macroscopic experiments.
    • Theoretical models are essential for interpreting diffusion in complex membrane environments.