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Updated: Jul 31, 2026
Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
Published on: March 5, 2017
A method of estimating radial distribution function of protein particles in membranes from freeze-fracture electron
Abstract:
A computational method for estimating the radial distribution function of particles seen in electron micrographs of freeze-fractured membranes is presented. The method overcomes the previous difficulty of measurements of this quantity from freeze-fracture electron micrographs--the systematic error due to the finite size of the sample areas in the freeze-fracture. By multiplying the measurements by weights specific to the shape of the sample area, the method lessens the errors due to the boundaries of the area and allows a synthesis of the average radial distribution function from counts over many small separate areas.
