Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Transfer RNA in reticulocyte maturation.

D W Smith, W C Weinberg

    Biochimica Et Biophysica Acta
    |September 28, 1981
    PubMed
    Summary

    Transfer RNA (tRNA) persists longer than ribosomes during rabbit reticulocyte maturation after hemolysis. Most amino acid tRNAs show uniform degradation, with a half-life of 50-60 hours.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Cancer mortality at very old ages.

    Cancer·1996
    Same author

    Racial differences on psychological measures in combat veterans seeking treatment for PTSD.

    Journal of personality assessment·1996
    Same author

    Mosquito-borne viruses and epidemic polyarthritis.

    The Medical journal of Australia·1996
    Same author

    Consensus phylogeny of Dictyostelium.

    Experientia·1995
    Same author

    Role of ventrolateral medulla catecholamine cells in hypothalamic neuroendocrine cell responses to systemic hypoxia.

    The Journal of neuroscience : the official journal of the Society for Neuroscience·1995
    Same author

    Venous catheter thrombus formation and pulmonary embolism in children.

    Pediatric pulmonology·1995

    Area of Science:

    • Molecular Biology
    • Cell Biology
    • Biochemistry

    Background:

    • Reticulocytes are immature red blood cells crucial for protein synthesis.
    • Phenylhydrazine-induced hemolysis is a model for studying red blood cell maturation and stress.
    • Transfer RNA (tRNA) plays a vital role in protein translation.

    Purpose of the Study:

    • To investigate the degradation kinetics of tRNA during rabbit reticulocyte maturation.
    • To compare the persistence of tRNA with other cellular components like ribosomes.
    • To determine the half-life of tRNA in maturing reticulocytes.

    Main Methods:

    • Comparing tRNA content in reticulocytes and mature erythrocytes.
    • Assessing the ability of cells to incorporate amino acids into protein.
    • Analyzing the integrity of the 3'-terminal pCpCpA moiety of tRNA.

    Main Results:

    • tRNA persists longer than ribosomes and protein synthesis capacity during reticulocyte maturation.
    • Uniform degradation of tRNA was observed, with approximately 15% remaining for most amino acids.
    • The estimated half-life of tRNA in maturing cells is 50-60 hours.
    • Little tRNA lacking the 3'-terminal pCpCpA was found in mature erythrocytes.

    Conclusions:

    • tRNA exhibits remarkable stability during the terminal stages of reticulocyte maturation.
    • The degradation pattern of tRNA is relatively uniform across different amino acids.
    • The findings provide insights into the molecular mechanisms governing red blood cell aging and component turnover.

    Related Experiment Videos