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Repeating structure of cytoplasmic poly(A)-ribonucleoprotein.

B W Baer, R D Kornberg

    Proceedings of the National Academy of Sciences of the United States of America
    |April 1, 1980
    PubMed
    Summary

    Cytoplasmic poly(A)-ribonucleoprotein exhibits a repeating structure, revealed by T2 RNase digestion. This structure, formed in the cytoplasm, can be reconstituted using poly(A) and cytoplasmic factors.

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    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Background:

    • Poly(A)-ribonucleoprotein complexes (PAPs) play crucial roles in mRNA regulation.
    • The structural organization of cytoplasmic PAPs is not fully understood.

    Purpose of the Study:

    • To investigate the structural organization of cytoplasmic poly(A)-ribonucleoprotein.
    • To determine the factors involved in the formation of this structure.

    Main Methods:

    • Digestion of cytoplasmic poly(A)-ribonucleoprotein with T2 RNase.
    • Reconstitution experiments using purified poly(A) and cytoplasmic factors.
    • Competition assays with various nucleic acids.

    Main Results:

    • T2 RNase digestion revealed a repeating structural unit of approximately 27 residues within cytoplasmic poly(A)-ribonucleoprotein.
    • The repeating structure was successfully reconstituted from purified poly(A) and cytoplasmic factors.
    • Reconstitution was specific for poly(A), with no competition observed from poly(G), poly(C), poly(dA), or tRNA.
    • The repeating structure was found to be absent in the nucleus, suggesting its formation occurs post-nuclear export.

    Conclusions:

    • Cytoplasmic poly(A)-ribonucleoprotein possesses a regular, repeating structure.
    • This structure is formed in the cytoplasm and involves specific interactions with poly(A).
    • The findings provide insights into the molecular organization and potential functions of cytoplasmic PAPs.

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