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Genetic control of murine IgD structural heterogeneity
Summary
Mouse B lymphocytes have two forms of surface immunoglobulin D (IgD): IgDI and IgDII. The ratio of these IgD forms varies between mouse strains and is genetically linked to the Igh-5 allotype.
Area of Science:
- Immunology
- Genetics
- Molecular Biology
Background:
- Murine B lymphocytes express two distinct forms of surface immunoglobulin D (IgD): IgDI and IgDII.
- IgDI comprises two delta heavy chains and two light chains, while IgDII consists of a single heavy and light chain.
Purpose of the Study:
- To investigate the variations in the ratio of IgDI to IgDII on mouse spleen cells.
- To determine the genetic basis underlying the differential expression of IgD forms.
Main Methods:
- Analysis of surface IgD forms on spleen cells from different mouse strains.
- Genetic linkage studies to identify the locus controlling the IgDI/IgDII ratio.
Main Results:
- Significant variations in the IgDI/IgDII ratio were observed among different mouse strains.
- Genetic evidence established a link between the IgDI/IgDII ratio and the Igh-5 allotype.
- Mice with Igh-5e allotype exhibit a low ratio, whereas Igh-5a or Igh-5b allotypes show a high ratio.
Conclusions:
- The IgDI/IgDII ratio in mice is a genetically determined trait linked to the Igh-5 allotype.
- The Igh-5 locus and the gene regulating the IgDI/IgDII ratio are located between the Igh-6 and Igh-V loci.