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Intraocular irrigating and replacement fluid

M V Graham, S Hodson

    Transactions of the Ophthalmological Societies of the United Kingdom
    |July 1, 1980
    PubMed
    Summary
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    Researchers studied the human corneal endothelium

    Area of Science:

    • Ophthalmology
    • Cell Physiology

    Background:

    • Corneal transparency is crucial for vision.
    • The corneal endothelium regulates hydration via a bicarbonate ion pump.
    • Electrical and fluid-flow methods can assess endothelial function.

    Purpose of the Study:

    • To evaluate methods for measuring human corneal endothelial function.
    • To investigate the effects of aqueous media on the human corneal endothelium.
    • To develop a trauma-free medium for corneal endothelium research.

    Main Methods:

    • Direct electrical measurements (current, potential, resistance) of endothelial ion transport.
    • Indirect fluid flow measurements using pachymetry or specular microscopy.
    • Assessment of human corneal endothelium viability in various aqueous media.

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    Main Results:

    • Electrical measurements in human corneas are reliable and comparable to rabbit data.
    • Specular microscopy can yield unreliable results in human corneas due to the scleral clamping phenomenon.
    • A novel aqueous medium was developed that minimizes trauma to the human corneal endothelium.

    Conclusions:

    • Electrical methods provide reliable assessment of human corneal endothelial function.
    • Careful selection of experimental media is essential for studying the human corneal endothelium.
    • The developed aqueous medium supports human corneal endothelium viability without causing trauma.