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Friend disease in vitro

T M Dexter, T D Allen, N G Testa

    The Journal of Experimental Medicine
    |September 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    This study details a novel in vitro system for generating Friend virus-induced erythroid cell lines. It establishes a reproducible method for isolating these cell lines, crucial for studying erythropoiesis and viral oncogenesis.

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    Area of Science:

    • Hematology
    • Virology
    • Cell Biology

    Background:

    • Erythropoiesis is normally suppressed at primitive levels in long-term marrow cultures.
    • Friend virus infection, specifically a complex of F-MuLV and SFFVp, disrupts normal hemopoiesis.

    Purpose of the Study:

    • To develop a complete in vitro system for reproducible production and isolation of Friend virus-induced erythroid cell lines.
    • To investigate erythropoietin-independent erythropoiesis and the generation of primitive erythroid cells.

    Main Methods:

    • Utilized long-term marrow cultures infected with a Friend virus complex (F-MuLV and SFFVp).
    • Induced erythropoiesis using anemic mouse serum prior to viral infection.
    • Passaged cells in a "virgin" marrow environment to establish permanent cell lines.

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    Main Results:

    • Viral infection led to elevated erythroid progenitor cells (CFU-E) and transient erythropoietin-independent erythropoiesis.
    • Sustained production of primitive erythroid cells with low hemoglobinization was observed.
    • Permanent, autonomous erythroid cell lines were established after serial passage in a marrow environment.

    Conclusions:

    • This system represents the first complete in vitro method for isolating Friend virus-induced erythroid cell lines.
    • The findings provide a valuable tool for studying erythroid differentiation and Friend virus-induced leukemogenesis.