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Nucleosome arcs and helices

J Dubochet, M Noll

    Science (New York, N.Y.)
    |October 20, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Electron microscopy reveals nucleosome core structures, including arcs and cylinders. These studies show nucleosome cores are wedge-shaped and exhibit conformational variability, possibly due to a rotational pivot.

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    Area of Science:

    • Structural biology
    • Molecular biophysics

    Background:

    • Nucleosome cores form regular crystalline arrangements.
    • Understanding nucleosome structure is crucial for chromatin organization.

    Purpose of the Study:

    • To analyze the structure of nucleosome cores using electron microscopy.
    • To elucidate the arrangement and properties of nucleosome crystals.

    Main Methods:

    • Electron microscopy of crystallized nucleosome cores.
    • Analysis of nucleosome arcs and cylinders.

    Main Results:

    • Identified two main regular structures: nucleosome arcs and cylinders.
    • Nucleosome cylinders consist of concentric cylindrical layers of right-handed helices.

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  • Determined nucleosome core shape as a short, wedge-shaped cylinder (approx. 110x110x60 Å).
  • Observed primary interaction between top and bottom planes of nucleosome cores.
  • Demonstrated significant conformational variability in nucleosome cores.

    • Conclusions:

    • Nucleosome cores possess a defined, albeit variable, structure.
    • Conformational flexibility is a key property of nucleosome cores.
    • A postulated pivot near the 70th base pair explains rotational freedom and variability.