Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Active gene sequences are undermethylated

T Naveh-Many, H Cedar

    Proceedings of the National Academy of Sciences of the United States of America
    |July 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Multiple pathways for high voltage-activated ca(2+) influx in anterior pituitary lactotrophs and somatotrophs.

    Journal of neuroendocrinology·2012
    Same author

    Gene repression paradigms in animal cells.

    Cold Spring Harbor symposia on quantitative biology·2005
    Same author

    Epigenetic crosstalk.

    Molecular cell·2001
    Same author

    Asynchronous replication and allelic exclusion in the immune system.

    Nature·2001
    Same author

    Developmental regulation of DNA replication timing at the human beta globin locus.

    The EMBO journal·2001
    Same author

    An upstream repressor element plays a role in Igf2 imprinting.

    The EMBO journal·2001
    Same journal

    The TaMYB55-TaSnRK1α1-TabZIP9 module confers heat stress tolerance in wheat.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Superstatistics approach to turbulent circulation fluctuations.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    A molecular timescale for evolution of cobamide biosynthesis.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Pierre Chambon, a pioneer of molecular biology and gene regulation in eukaryotes.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Granulosa cell glycogen fuels the avascular corpus luteum.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Synthetic essentiality of TRAIL/TNFSF10 in VHL-deficient renal cell carcinoma.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    See all related articles

    Active genes show less DNA methylation. This study reveals that actively transcribed genes have significantly lower methylation levels compared to the overall genome, suggesting undermethylation is key to gene activity.

    Area of Science:

    • Molecular Biology
    • Epigenetics
    • Genomics

    Background:

    • Chromosome methylation patterns are crucial for gene regulation.
    • Active genomic regions exhibit distinct epigenetic marks.
    • Understanding DNA methylation in active vs. inactive chromatin is essential.

    Purpose of the Study:

    • To investigate the degree of DNA methylation in active chromosomal regions.
    • To compare methylation levels in DNase I-sensitive regions with the overall genome.
    • To determine if undermethylation is characteristic of actively transcribed genes.

    Main Methods:

    • Utilized DNase I to label active nuclear regions.
    • Employed methylation-specific restriction enzymes (Hpa II, Hha I) to probe DNA.
    • Quantified CpG methylation using nick-translation with [alpha-32P]dGTP and nearest-neighbor analysis.

    Related Experiment Videos

    Main Results:

    • Approximately 70% of all CpG sequences in animal cell DNA are methylated.
    • Active, DNase I-sensitive regions show lower methylation (30-40% of CpGs).
    • Expressed gene sequences complementary to cellular RNA are only 20-30% methylated.

    Conclusions:

    • Undermethylation is a general characteristic of actively transcribed genes.
    • Differential DNA methylation plays a significant role in gene regulation.
    • Epigenetic modifications like methylation are critical for controlling gene expression.