Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Conversion of B DNA between solution and fiber conformations

M Mandelkern, N Dattagupta, D M Crothers

    Proceedings of the National Academy of Sciences of the United States of America
    |July 1, 1981
    PubMed
    Summary

    Rodlike DNA molecules form bundles at low salt and high concentrations. These DNA aggregates can be reversed with EDTA or induced by trivalent ions, showing B-form optical anisotropy.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Heterogeneity of anatomic regions by MR volumetry in juvenile myoclonic epilepsy.

    Acta neurologica Scandinavica·2016
    Same author

    Study of CP asymmetry in B^{0}-B[over ¯]^{0} mixing with inclusive dilepton events.

    Physical review letters·2015
    Same author

    Search for a dark photon in e(+)e(-) collisions at BABAR.

    Physical review letters·2014
    Same author

    Search for CP violation in B0-B0 mixing using partial reconstruction of B0→D*- Xℓ+ νℓ and a kaon tag.

    Physical review letters·2014
    Same author

    Human brain metabolic responses to familiarity during lexical decision.

    Human brain mapping·2014
    Same author

    Measurement of the D*(2010)+ meson width and the D*(2010)+ - D0 mass difference.

    Physical review letters·2013

    Area of Science:

    • Molecular Biology
    • Biophysics
    • Materials Science

    Background:

    • DNA molecules can exhibit different structural arrangements depending on environmental conditions.
    • Understanding DNA aggregation is crucial for fields like nanotechnology and gene delivery.

    Purpose of the Study:

    • To investigate the conditions and mechanisms of DNA molecule aggregation.
    • To characterize the structural and optical properties of DNA aggregates.

    Main Methods:

    • Controlled manipulation of salt concentration and addition of specific ions (EDTA, trivalent ions).
    • Observation of DNA molecule aggregation and bundle formation.
    • Measurement of optical anisotropy of aggregated DNA species.

    Main Results:

    • Rodlike DNA molecules form specific parallel bundles at low salt and high concentrations.
    • Bundle formation is reversible upon addition of EDTA.
    • Trivalent ions can induce aggregate formation at higher salt concentrations.
    • Aggregated DNA exhibits optical anisotropy consistent with the B-form DNA structure.

    Conclusions:

    • DNA molecule aggregation is a salt- and ion-dependent phenomenon.
    • The aggregated state of DNA possesses distinct structural and optical properties compared to monomeric DNA.
    • These findings have implications for controlling DNA structure and function in vitro.

    Related Experiment Videos