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Intercellular recognition: quantitation of initial binding events

D R McClay, G M Wessel, R B Marchase

    Proceedings of the National Academy of Sciences of the United States of America
    |August 1, 1981
    PubMed
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    This study quantifies intercellular binding strengths using a novel cell assay. It reveals two distinct stabilization processes in cell adhesion, one metabolic-dependent and one independent.

    Area of Science:

    • Cell Biology
    • Biophysics
    • Developmental Biology

    Background:

    • Intercellular adhesion is crucial for tissue development and function.
    • Understanding the forces and mechanisms governing cell adhesion is essential.

    Purpose of the Study:

    • To quantitatively measure intercellular binding strengths.
    • To investigate the hypothesis that cell adhesion involves distinct recognition and stabilization phases.
    • To characterize the nature of cell adhesion stabilization processes.

    Main Methods:

    • Development of a quantitative cell binding assay using radioactive single cells and centrifugal force.
    • Measurement of dislodgment force for embryonic chicken neural retina cells from monolayers.
    • Analysis of time- and temperature-dependent stabilization processes.

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    Main Results:

    • Intercellular binding forces were quantified at approximately 10(-5) dyne.
    • Topographic specificities in cell adhesion were detected.
    • Two distinct stabilization processes were identified: one metabolic-dependent (increasing binding 13-fold) and one metabolic-independent (increasing binding 2-fold).
    • The metabolic-independent process showed temperature dependence similar to membrane diffusion.

    Conclusions:

    • The study supports the hypothesis of separable recognition and stabilization events in intercellular adhesion.
    • Two distinct stabilization mechanisms contribute to the overall strength of cell adhesion.
    • These findings provide quantitative insights into the biophysical forces governing cell-cell interactions.