Characteristics of two anti-tumour monoclonal antibody preparations

Monoclonal antibodies directed against one spontaneously arising rat mammary carcinoma and a human osteogenic sarcoma have been prepared following the fusion of spleen cells from appropriately immunised donors with mouse myeloma cells. The characteristics of these antibodies have been analysed and methods for their purification have been developed using immunoadsorption chromatography or by utilising their affinity for Sepharose-linked Protein A. The use of these antibodies for the identification, characterisation and isolation of their target tumour antigenic structures is described.