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A monoclonal antibody specific for mouse dendritic cells
Summary
A new antibody, 33D1, specifically targets dendritic cells (DC), a rare immune cell type. This discovery enables accurate monitoring of dendritic cell populations in complex biological samples.
Area of Science:
- Immunology
- Cell Biology
Background:
- Dendritic cells (DC) are crucial immune cells with unique characteristics.
- Identifying and quantifying DC within complex lymphoid mixtures is challenging.
Purpose of the Study:
- To develop and characterize a novel antibody specific for dendritic cells.
- To establish a method for monitoring dendritic cell content.
Main Methods:
- Generation of monoclonal antibody 33D1 by fusing immune rat spleen cells with myeloma.
- Screening hybridoma supernatants against dendritic cells, macrophage cell lines, and lymphoblasts.
- Quantitative binding studies using radiolabeled 33D1 antibody and inhibition assays.
Main Results:
- Antibody 33D1 specifically bound to and killed 80-90% of dendritic cells from spleen and lymph nodes.
- No significant binding or killing was observed in other leukocytes, including macrophages.
- Dendritic cells expressed the 33D1 antigen stably in culture, unlike macrophages and lymphocytes.
Conclusions:
- The 33D1 antibody identifies a stable and specific antigen on dendritic cells.
- 33D1 antibody is a valuable tool for quantifying dendritic cells in heterogeneous cell populations.