Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Physicochemical and functional changes in human leukemic cell line HL-60

O Stendahl, C Dahlgren, J Hed

    Journal of Cellular Physiology
    |August 1, 1982
    PubMed
    Summary

    Dimethyl sulfoxide (DMSO) induces differentiation in human HL-60 cells, enhancing phagocytic activity and chemiluminescence (CL) responses. Fc receptor function matures earlier than C3b and PMA pathways.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Sedimentation rate and suPAR in relation to disease activity and mortality in patients with tuberculosis.

    The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2019
    Same author

    Effects of albendazole on the clinical outcome and immunological responses in helminth co-infected tuberculosis patients: a double blind randomised clinical trial.

    International journal for parasitology·2014
    Same author

    Shortening the 'short-course' therapy- insights into host immunity may contribute to new treatment strategies for tuberculosis.

    Journal of internal medicine·2013
    Same author

    Effects of a food supplement rich in arginine in patients with smear positive pulmonary tuberculosis--a randomised trial.

    Tuberculosis (Edinburgh, Scotland)·2011
    Same author

    What formyl peptide receptors, if any, are triggered by compound 43 and lipoxin A4?

    Scandinavian journal of immunology·2011
    Same author

    Lipoxin A(4) metabolites/analogues from two commercial sources have no effects on TNF-alpha-mediated priming or activation through the neutrophil formyl peptide receptors.

    Scandinavian journal of immunology·2009

    Area of Science:

    • Hematology
    • Cell Biology
    • Immunology

    Background:

    • Human promyelocytic cell line HL-60 is a model for studying myeloid differentiation.
    • Dimethyl sulfoxide (DMSO) is a known inducer of differentiation in various cell types.

    Purpose of the Study:

    • To investigate the physicochemical and functional changes during DMSO-induced differentiation of HL-60 cells.
    • To determine the temporal development of different functional responses during differentiation.

    Main Methods:

    • HL-60 cells were cultured with DMSO to induce differentiation.
    • Physicochemical properties were assessed using a hydrophobic two-phase system.
    • Functional assays included chemiluminescence (CL) response and phagocytosis using IgG- or C3b-opsonized yeast particles and phorbol 12-myristate 13-acetate (PMA).

    Related Experiment Videos

    Main Results:

    • DMSO treatment led to decreased cell volume and increased hydrophobic interaction.
    • Mature granulocytes were separated from immature cells using a hydrophobic system.
    • Chemiluminescence (CL) and phagocytic activity increased with DMSO exposure.
    • Fc receptor-mediated responses (IgG opsonization) showed earlier enhancement than C3b or PMA-induced responses.

    Conclusions:

    • DMSO induces differentiation with distinct physicochemical and functional changes in HL-60 cells.
    • Fc receptor-mediated phagocytosis and oxidative metabolism activation precede C3b and PMA pathways.
    • Differential activation kinetics suggest distinct mechanisms for Fc, C3b, and PMA stimulation.