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Related Experiment Videos

Perforated postsynaptic densities: probable intermediates in synapse turnover

M Nieto-Sampedro, S F Hoff, C W Cotman

    Proceedings of the National Academy of Sciences of the United States of America
    |September 1, 1982
    PubMed
    Summary
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    Perforated postsynaptic densities (PSDs) in rat dentate gyrus spines are key to synapse turnover. Their numbers dynamically change, suggesting a role in nervous system maintenance and adaptation.

    Area of Science:

    • Neuroscience
    • Cell Biology
    • Synaptic Plasticity

    Background:

    • The molecular layer of the dentate gyrus exhibits numerous perforated postsynaptic densities (PSDs).
    • These perforations are primarily observed in complex PSDs with U- or W-shaped profiles.
    • Perforated PSDs constitute a significant proportion of complex PSDs in both young and aged rats.

    Purpose of the Study:

    • To investigate the dynamic changes in perforated PSDs during synapse turnover.
    • To explore the relationship between perforated and nonperforated PSDs in the dentate gyrus.
    • To understand the role of PSDs in nervous system maintenance and adaptation.

    Main Methods:

    • Induction of synapse turnover via ipsilateral entorhinal cortex ablation in rats.
    • Quantitative analysis of perforated and nonperforated PSDs in the dentate gyrus molecular layer at various time points postlesion.

    Related Experiment Videos

  • Morphological characterization of PSDs, distinguishing between simple and complex types.

    • Main Results:

    • Synapse turnover significantly alters PSD composition: simple PSDs decrease while perforated PSDs increase initially.
    • A dynamic inverse relationship exists between nonperforated and perforated PSDs, suggesting a precursor-product relationship.
    • PSD proportions gradually return to normal levels by 120 days postlesion.

    Conclusions:

    • Perforated PSDs are likely intermediates in a continuous synapse turnover cycle.
    • This turnover process is integral to the normal maintenance and adaptive capabilities of the nervous system.
    • The findings provide insights into the structural plasticity of synapses in the dentate gyrus.