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Related Experiment Videos

Alternate g-g- conformation for dinucleoside phosphates in solution

C H Lee, N E Sharpless, W H Jennings

    Biophysical Chemistry
    |November 1, 1982
    PubMed
    Summary

    Researchers identified a new dinucleoside conformation (conformer I') in solution. This discovery reveals four stable dinucleoside conformers and suggests potential interconversion between vertical and regular double helices.

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    Area of Science:

    • Biochemistry
    • Structural Biology
    • Molecular Biophysics

    Background:

    • Dinucleosides exist in various conformations in solution.
    • Understanding these conformations is crucial for nucleic acid structure and function.

    Purpose of the Study:

    • To identify and characterize alternative conformations of dinucleosides in solution.
    • To investigate the relationship between different dinucleoside conformers and double helix structures.

    Main Methods:

    • Computational analysis using potential energy calculations.
    • Experimental validation through nuclear magnetic resonance (NMR) spectroscopy.

    Main Results:

    • A novel g-g- conformation (conformer I") for dinucleosides was identified.

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  • Conformer I" exhibits larger glycosidic torsional angles (chi = 94-111°) compared to conformer I (chi = 8-35°).
  • Four stable dinucleoside conformers (I, I', II, and III) are in equilibrium with open forms.

    • Conclusions:

    • The structure of conformer I" supports the 'vertical' double helix model.
    • Findings suggest potential interconversion pathways between vertical and regular double helices (A-form DNA/RNA, A"-form RNA).