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[Fast alpha 1-antitrypsin phenotyping for the differentiation of PiMM-sub-types (author's transl)]
The polyacrylamide gel slabs for isoelectric focussing were adapted for 20 microliter samples by adding a certain quantity of urea, and by letting it run idle before adding the sample. A better separation of protein fractions was obtained, and the distance of the M2- and M8-bands was increased to more than 20 mm in a total migration length of 120 mm. A M1M3 standard serum was introduced and the M8 band was included to differentiate PiMM sub-types. The clinical use of the method is demonstrated with the results of a recently published clinical study by Bencze, K. et al. ((1979), Chest 76, in press).
The polyacrylamide gel slabs for isoelectric focussing were adapted for 20 microliter samples by adding a certain quantity of urea, and by letting it run idle before adding the sample. A better separation of protein fractions was obtained, and the distance of the M2- and M8-bands was increased to more than 20 mm in a total migration length of 120 mm. A M1M3 standard serum was introduced and the M8 band was included to differentiate PiMM sub-types. The clinical use of the method is demonstrated with the results of a recently published clinical study by Bencze, K. et al. ((1979), Chest 76, in press).