[Analysis of lactate dehydrogenase isoenzymes in murine lymphocyte populations]

The pattern of lactate dehydrogenase isoenzyme has been examined for different murine lymphoid cell populations and for T and B lymphocyte populations. LDH-1, LDH-2, LDH-3, LDH-4 and LDH-5 were found in the thymus, LDH-3, LDH-4 and LDH-5 in the spleen, and LDH-4 and LDH-5 in lymph nodes. Enrichment of lymphocytes with T and B cells resulted in the appearance of LDH-4 and LDH-5 in T cell population, and of LDH-5 in B cell population. With the appearance of the autoimmune reaction, activity of LDH-5 in the thymus was seen to decrease. After the thymosin injection (4 fraction), activities of LDH-1, LDH-2 and LDH-3 diminished. A possible application of the LDH isoenzyme distribution pattern as a marker of T cell differentiation is discussed.