Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microgranulocytotoxicity

J W Blaschke, C D Severson, N E Goeken

    The Journal of Laboratory and Clinical Medicine
    |August 1, 1977
    PubMed
    Summary

    Researchers refined microcytotoxicity assays using granulocytes to identify neutrophil-specific antigens. This novel technique, employing double-fluorescent vital staining, revealed specificities relevant to transplantation and transfusion reactions.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Pulmonary vasodilation in the rat by insulin in vitro could indicate potential hazard for inhaled insulin.

    Diabetologia·2003
    Same author

    Altered response to adaptive signals in transplanted intestine.

    Transplantation proceedings·2002
    Same author

    Measurement of the electron affinity of cerium.

    Physical review letters·2002
    Same author

    Nutritional effects of surgical and medical treatment for short bowel syndrome.

    JPEN. Journal of parenteral and enteral nutrition·2001
    Same author

    Effect of epidural anesthesia and analgesia on perioperative outcome: a randomized, controlled Veterans Affairs cooperative study.

    Annals of surgery·2001
    Same author

    BAFF-R, a newly identified TNF receptor that specifically interacts with BAFF.

    Science (New York, N.Y.)·2001

    Area of Science:

    • Immunology
    • Hematology
    • Cellular Biology

    Background:

    • Microcytotoxicity assays are crucial for identifying cell-specific antigens.
    • Previous methods using granulocytes as target cells required significant refinement for reproducibility.
    • Neutrophil-specific antigens have implications in transfusion reactions and immune disorders.

    Purpose of the Study:

    • To develop and validate a refined microcytotoxicity assay for identifying neutrophil-specific antigens.
    • To characterize the nature of these neutrophil-specific antigens and their relationship to known immune systems.
    • To explore the clinical relevance of these antigens in transplantation and transfusion medicine.

    Main Methods:

    • Extensive revision of the microcytotoxicity assay technique.
    • Adoption of double-fluorescent vital staining for detecting cytotoxicity.
    • Utilizing granulocytes as target cells and characterizing antigen reactivity with alloantisera and complement.

    Main Results:

    • The revised assay provided clear, reproducible results for identifying neutrophil-specific antigens.
    • Detected antigens did not correlate with established human histocompatibility systems (HLA, ABH, NA, NB, NC) or other blood cell types.
    • Cytotoxicity was specific, mediated by immunoglobulin and complement, confirming an antigen-antibody reaction.

    Conclusions:

    • The refined assay successfully identifies neutrophil-specific antigens.
    • These antigens are distinct from known histocompatibility antigens and are found on neutrophils.
    • Identified specificities may influence bone marrow transplantation, febrile transfusion reactions, and immunoneutropenias.

    Related Experiment Videos