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Some functional properties of the RNP nucleoplasmic antigen
Scandinavian Journal of Immunology
|January 1, 1980
Summary
Nuclear stimulators increase nuclear antigens RNP and Sm in lymphocytes. Alpha-amanitin inhibits RNP accumulation, suggesting RNP and Sm are active participants in nuclear functions.
Area of Science:
- Cell Biology
- Immunology
- Molecular Biology
Background:
- Nucleoplasmic antigens (ENA) play roles in cellular processes.
- Understanding ENA regulation is crucial for cell biology and immunology.
Purpose of the Study:
- To investigate the effects of nuclear stimulators and inhibitors on ENA.
- To determine the dynamic nature of RNP and Sm antigens within the nucleus.
Main Methods:
- Indirect immunofluorescence technique to visualize ENA.
- Utilized human autoimmune sera for RNP and Sm antigen detection.
- Employed various mitogens (phytohaemagglutinin, concanavalin A, pokeweed mitogen) and alpha-amanitin.
Main Results:
- Mitogens induced accumulation of RNP and Sm antigens in lymphocytes.
- Accumulation was primarily in B cells with certain mitogens.
- Alpha-amanitin inhibited RNP accumulation and reduced RNP levels in cell lines.
- Sm antigen levels were unaffected by alpha-amanitin.
Conclusions:
- RNP and Sm nucleoplasmic antigens are dynamic, rapid-phase reactants.
- These antigens are integrated into physiological nuclear mechanisms.
- Evidence suggests they are not inert structural components or viral products.