Ristocetin-Willebrand factor activity in dog plasma

A sensitive and reproducible method for the quantitation of ristocetin-Willebrand factor activity in canine plasma is described. This assay measures the initial velocity of aggregation of suspensions of canine washed platelets in the presence of ristocetin and ristocetin-Willebrand factor. The washed platelets are stable for 4 h and when prepared from the same donor vary little in their day-to-day response to ristocetin. The calculated mean plasma ristocetin-Willebrand factor activity in 37 normal dogs using this method is 98 +/- 26% (mean +/- SD) Ristocetin-Willebrand activity is 106 +/- 25% in dogs with severe hemophilia A and 45 +/- 13% in dogs with moderate forms of von Willebrand's disease.