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[Immunosorption isolation of Thy-1 antigen from thymocyte plasma membrane components]
CBA-thymocyte receptors responsible for autologous rosette formation were found to inhibit specific cytotoxicity of antibrain antibody to thymocytes. The receptors isolated by heating at 45 degrees C in isotonic solution were investigated by chromatography on immobilized antibrain antibody, after absorption on the column with immobilized normal rabbit IgG. Three fractions were obtained. Only one of the fractions eluted from the immobilized antibrain antibody was found to inhibit cytotoxicity of rabbit anti-CBA-brain antibody for CBA thymocytes. It is supposed that the antibrain antibodies have an affinity not only to antigen Thy-1 but also to other structures of the thymocyte plasma membrane.
CBA-thymocyte receptors responsible for autologous rosette formation were found to inhibit specific cytotoxicity of antibrain antibody to thymocytes. The receptors isolated by heating at 45 degrees C in isotonic solution were investigated by chromatography on immobilized antibrain antibody, after absorption on the column with immobilized normal rabbit IgG. Three fractions were obtained. Only one of the fractions eluted from the immobilized antibrain antibody was found to inhibit cytotoxicity of rabbit anti-CBA-brain antibody for CBA thymocytes. It is supposed that the antibrain antibodies have an affinity not only to antigen Thy-1 but also to other structures of the thymocyte plasma membrane.