Related Experiment Videos
Preparation of colony stimulating activity from human leukemic urine: hydrophobic chromatography on
Biomedicine / [Publiee Pour L'A.A.I.C.I.G.]
|January 1, 1980
Abstract:
Urinary colony stimulating factor (CSF) from human leukemic urine is retained on propylamine-agarose hydrophobic chromatography; about 70% of total activity is eluted by the addition of 1 M NaCl with significant increase in specific activity. Incomplete binding of urinary CSF to this resin further confirm that biological activity is a heterogeneous pool of different molecular species. Analysis of the macro- and microscopic composition of colonies indicates that CSF resulting from this purification step maintains the ability to stimulate both granulocytic and macrophage progenitors.