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Functional subsets of B cells defined by quantitative differences in surface I-A

J L Greenstein, E M Lord, P Horan

    Journal of Immunology (Baltimore, Md. : 1950)
    |June 1, 1981
    PubMed
    Summary
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    Surface I-A expression levels on B cells correlate with their functional responses. B cells with low I-A expression are uniquely capable of responding to hapten coupled to Ficoll, indicating functional heterogeneity.

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • B cell heterogeneity influences immune responses.
    • Surface I-A determinants play a role in B cell function.

    Purpose of the Study:

    • To investigate the relationship between quantitative expression of surface I-A on B cells and their functional responsiveness.
    • To define B cell heterogeneity based on I-A expression levels and hapten-carrier responsiveness.

    Main Methods:

    • B cells were stained with fluorescein-conjugated anti-I-Ak antibody.
    • Cells were sorted into high and low I-A expressing populations using a fluorescence-activated cell sorter.
    • Anti-TNP precursor frequency was determined by limiting dilution analysis against TNP-lipopolysaccharide, TNP-sheep red blood cells, and TNP-Ficoll.

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    Main Results:

    • B cells responsive to TNP-lipopolysaccharide and TNP-sheep red blood cells were found in both low and high I-A expressing populations.
    • B cells responsive to TNP-Ficoll were exclusively identified within the low I-A expressing B cell population.

    Conclusions:

    • Quantitative expression of surface I-A can distinguish functional B cell subsets.
    • B cell populations with low surface I-A expression possess unique responsiveness to hapten-Ficoll conjugates.