Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Interleukin 2 in cell-mediated immune responses

V Paetkau, J Shaw, B Caplan

Journal of Supramolecular Structure

|January 1, 1980

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

MiniBooNE and MicroBooNE Combined Fit to a 3+1 Sterile Neutrino Scenario.

Physical review letters·2022

Same author

Retraction Note to: The Akt inhibitor KP372-1 suppresses Akt activity and cell proliferation and induces apoptosis in thyroid cancer cells.

British journal of cancer·2021

Same author

Expanded analysis of secondary germline findings from matched tumor/normal sequencing identifies additional clinically significant mutations.

JCO precision oncology·2019

Same author

Measurements of Nonsinglet Moments of the Nucleon Structure Functions and Comparison to Predictions from Lattice QCD for Q^{2}=4 GeV^{2}.

Physical review letters·2019

Same author

The Pandora multi-algorithm approach to automated pattern recognition of cosmic-ray muon and neutrino events in the MicroBooNE detector.

The European physical journal. C, Particles and fields·2019

Same author

Ultra-deep next-generation sequencing of plasma cell-free DNA in patients with advanced lung cancers: results from the Actionable Genome Consortium.

Annals of oncology : official journal of the European Society for Medical Oncology·2019

Interleukin 2 (IL2) can restore T cell responses in compromised immune systems. This study shows IL2 is crucial for maintaining cytotoxic T lymphocyte (CTL) activity and highlights other immune cell functions.

Area of Science:

Immunology
Cellular Biology

Background:

Interleukin 2 (IL2) is a lymphokine known to restore T cell responses.
UV irradiation of cells in mixed leukocyte cultures impairs T cell responses, including cytotoxic T lymphocyte (CTL) production and DNA synthesis.

Purpose of the Study:

To investigate the role of IL2 in restoring T cell responses after UV irradiation.
To determine the continuous requirement of IL2 for maintaining CTL activity.
To explore the functions of macrophages beyond IL2 production.

Main Methods:

Mixed leukocyte cultures (MLC) were used to assess T cell responses.
UV irradiation was applied to stimulating allogeneic cells.
IL2 was administered to evaluate its restorative effects.
Adherent-cell-depleted cultures were used to study macrophage functions.

Related Experiment Videos

Main Results:

IL2 restored both CTL production and DNA synthesis in UV-irradiated MLC.
UV-irradiated stimulators failed to induce normal IL2 production.
Continuous IL2 presence was necessary for maintaining CTL activity.
IL2 did not fully restore secondary responses in the absence of macrophages, suggesting other macrophage functions.

Conclusions:

IL2 plays a critical role in restoring and maintaining T cell-mediated immunity, particularly CTL responses.
Macrophages have essential functions beyond IL2 production, likely including antigen presentation.