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Helper activity of T lymphocytes which have been stimulated by keyhole limpet haemocyanin in vitro

K N Ward

    Immunology
    |May 1, 1981
    PubMed

    Details are given of a system for keyhole limpet haemocyanin (KLH)-induced DNA synthesis by murine T lymphocytes in vitro. Lymph node T cells from mice primed with KLH and Bordetella pertussis were stimulated with KLH under the defined conditions, and it was found that such cultured cells exhibited substantial non-specific helper activity. In contrast similarly primed T cells which had not been cultured showed only antigen-specific help. It is concluded that proper account should be taken of non-specific effects when studying the activity of antigen-specific helper cells in in vitro.

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