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Intracellular ionic activities in frog skin

W Nagel, J F Garcia-Diaz, W M Armstrong

    The Journal of Membrane Biology
    |January 1, 1981
    PubMed
    Summary
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    Frog skin epithelial cells actively accumulate chloride ions. Amiloride treatment alters membrane potential and intracellular sodium, but the driving force for sodium remains constant, suggesting sustained Na+–K+ ATP-ase pump activity.

    Area of Science:

    • Physiology
    • Cell Biology
    • Membrane Transport

    Background:

    • Frog skin epithelium is a model for studying ion transport.
    • Understanding active ion accumulation is crucial for epithelial function.
    • Amiloride is a known inhibitor of epithelial sodium channels.

    Purpose of the Study:

    • To measure intracellular ion activities and membrane potential in frog skin.
    • To investigate the effects of amiloride on these parameters.
    • To elucidate the role of the Na+–K+ ATP-ase pump in sodium transport.

    Main Methods:

    • Microelectrode impalement to measure intracellular Na+, K+, and Cl- activities (aiNa, aiK, aiCl).
    • Measurement of transapical membrane potential (V0) in isolated, short-circuited frog skins.

    Related Experiment Videos

  • Application of amiloride to the apical bathing medium.

    • Main Results:

    • Frog skin epithelial cells actively accumulate chloride (aiCl is 4.4 times equilibrium).
    • Amiloride initially hyperpolarized V0 without changing aiNa, aiK, or aiCl.
    • Long-term amiloride exposure decreased aiNa and depolarized V0, while the Na+ driving force remained constant.

    Conclusions:

    • Frog skin epithelial cells actively transport and accumulate chloride ions.
    • Amiloride affects membrane potential and intracellular sodium levels.
    • The Na+–K+ ATP-ase pump likely generates a maximal driving force for Na+ under amiloride exposure.