[Formation of the cell wall during the formation of Entamoeba invadens cysts]

The presence of chitin in the walls of E. invadens cysts has provided a new way to study the process of encystation, since chitin stains intensely in the presence of Calcofluor white M2R, a fluroescent dye. With this technique we have observed various stages in the differentiation of trophozoite membrane, then the cyst is formed inside the trophozoite and finally the cyst leaves the trophozoite after partial degradation of trophozoite body.