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[Changes in the detectability of HLA antigens on lymphocyte subpopulations induced by mitogens and corticosteroids]

A Iu Volgin, L A Pevnitskiĭ

    Biulleten' Eksperimental'Noi Biologii I Meditsiny
    |January 1, 1982
    PubMed

    A study was made of changes in the detectability of HLA-A, HLA-B and HLA-DR histocompatibility antigens on subpopulations of immunocompetent cells under the effect of mitogens and corticosteroids. HLA-antigens were identified by the lymphocytotoxic test. It was found that cell cultivation and mitogen stimulation lead to the fact that distinct reaction with the cells is produced by a greater number of HLA-antisera, while the specificity of direct and cross reactions remains unchanged. The main contribution to changes in the detectability of HLA-antigens is made by T cells. The exposure of the cells to dexamethasone in a dose producing no cytotoxic effect drastically lowered cell proliferation under the effect of PHA and deteriorated the detectability of HLA-A and HLA-B antigens. As regards HLA-DR antigens, it was disclosed that PM-stimulation of B cells considerably decreased the expression of their antigens, whereas PHA-stimulation of T cells led to the detection of HLA-DR antigens on these cells.

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