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Interleukin-2 does not induce murine B cells to secrete Ig
Journal of Immunology (Baltimore, Md. : 1950)
|December 1, 1982
Summary
Interleukin-2 (IL-2) does not directly induce B cell differentiation or immunoglobulin secretion. This finding clarifies the role of IL-2 in B cell responses, distinct from other lymphokines.
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- Two T cell-derived lymphokines, B cell differentiation factor mu (BCDF-μ) and B cell differentiation factor gamma (BCDF-γ), induce B cell differentiation without Interleukin-2 (IL-2).
- Previous studies suggested IL-2 was not required for immunoglobulin (Ig) secretion, but its direct effect on B cell differentiation remained unclear.
Purpose of the Study:
- To investigate the direct effect of purified IL-2 on B cell differentiation and Ig secretion.
- To determine if IL-2 influences Ig secretion induced by other factors.
Main Methods:
- Assessed the ability of purified IL-2 to induce IgM or IgG secretion in BCL1 tumor cells and normal B cells.
- Examined IL-2's effect on BCDF-μ-induced IgM secretion and lipopolysaccharide (LPS) or BCDF-γ-induced IgG secretion.
Main Results:
- IL-2 did not induce IgM secretion by BCL1 tumor cells.
- IL-2 did not enhance BCDF-μ-induced IgM secretion in these cells.
- IL-2 had no effect on LPS or BCDF-γ-induced IgG secretion in normal B cells.
Conclusions:
- IL-2 does not directly induce B cell differentiation.
- IL-2 does not directly induce IgM or IgG secretion by B cells.
- The function of IL-2 in B cell responses is not mediated by direct action on B cell differentiation.