Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The idiotypic network: the murine MOPC315 anti-DNP system

M Amor, B Mariamé, D Voegtlé

    Annales D'Immunologie
    |November 1, 1982
    PubMed
    Summary

    Mice immunized with an anti-idiotypic antibody produced two types of antibodies. Some antibodies connected to the idiotypic network, suggesting broader immune system connections beyond a single idiotype.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Videolaryngoscopy versus direct laryngoscopy for tracheal intubation by anesthesia residents in the operating room: The randomized multicenter VILARE trial protocol.

    Revista espanola de anestesiologia y reanimacion·2026
    Same author

    Impact of universal use of the McGrath videolaryngoscope as the first option for all intubations in the operating room: The multicentre prospective before-after VIDEOLAR-SURGERY study protocol.

    Revista espanola de anestesiologia y reanimacion..·2024
    Same author

    Common dysregulated pathways in obese adipose tissue and atherosclerosis.

    Cardiovascular diabetology·2016
    Same author

    Identification of matrix metalloproteinase-12 as a candidate molecule for prevention and treatment of cardiometabolic disease.

    Molecular medicine (Cambridge, Mass.)·2016
    Same author

    Genetic control of HgCl2-induced IgE and autoimmunity by a 117-kb interval on rat chromosome 9 through CD4 CD45RChigh T cells.

    Genes and immunity·2013
    Same author

    [A comparison of Airtraq™ laryngoscope and standard direct laryngoscopy in adult patients with immobilized cervical spine].

    Annales francaises d'anesthesie et de reanimation·2013

    Area of Science:

    • Immunology
    • Molecular Biology
    • Serology

    Background:

    • Myeloma proteins like MOPC315 possess unique idiotypic structures.
    • Anti-idiotypic antibodies can target specific idiotopes on other antibodies.
    • The MOPC315 immunoglobulin A (IgA) lambda 2 protein has a private idiotope dependent on heavy and light chain interaction.

    Purpose of the Study:

    • To investigate the immune response to a specific anti-idiotypic antibody (Id 315.1.4).
    • To characterize the antibodies generated against Id 315.1.4 in BALB/c mice.
    • To explore the connectivity of the idiotypic network.

    Main Methods:

    • Immunization of BALB/c mice with monoclonal anti-idiotypic antibody Id 315.1.4.
    • Characterization of resultant antibodies in mouse sera using binding assays.
    • Analysis of light chain types (kappa or lambda) and antigen-binding capabilities (anti-DNP function).

    Main Results:

    • Two distinct antibody populations were identified in mice immunized with Id 315.1.4.
    • Category 1: Antibodies with kappa light chains, lacking anti-DNP activity.
    • Category 2: Antibodies with lambda light chains, exhibiting DNP antigen-binding capacity.

    Conclusions:

    • The immune response to anti-idiotypic antibodies is complex and generates diverse antibody populations.
    • The idiotypic network appears interconnected, linking different idiotype systems.
    • The presence of lambda chain antibodies with DNP-binding function suggests connections between the MOPC315 idiotype system and other immune responses.

    Related Experiment Videos