Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Brain primary culture - a characterization

E Hansson, A Sellström, L I Persson

    Brain Research
    |April 21, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Critical appraisal of the "one-day-per-percent" rule - A register-based observational study.

    Burns : journal of the International Society for Burn Injuries·2024
    Same author

    A randomized, triple-blinded controlled clinical study with a novel disease-modifying drug combination in equine lameness-associated osteoarthritis.

    Osteoarthritis and cartilage open·2023
    Same author

    Nerve growth factor in the equine joint.

    Veterinary journal (London, England : 1997)·2020
    Same author

    Patients with unilateral transfemoral amputation treated with a percutaneous osseointegrated prosthesis: a cost-effectiveness analysis.

    The bone & joint journal·2018
    Same author

    Similar cost-utility for double- and single-bundle techniques in ACL reconstruction.

    Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA·2017
    Same author

    Long-term treatment with methylphenidate for fatigue after traumatic brain injury.

    Acta neurologica Scandinavica·2016
    Same journal

    IGFBP3 and UBE2C are associated with protein modification pathways and serve as prognostic markers in glioma.

    Brain research·2026
    Same journal

    Targeting neurodevelopmental miR132-3p promotes neuroprotection and axon regeneration after optic nerve injury in mice.

    Brain research·2026
    Same journal

    Variability in acoustic startle response and prepulse inhibition across adulthood in Fragile X messenger ribonucleoprotein 1 knockout mice.

    Brain research·2026
    Same journal

    Transcriptome-guided modeling reveals insulin-related metabolic dysfunction in SCA3 mouse cerebellum.

    Brain research·2026
    Same journal

    Intranasal stromal cell-derived factor-1α mitigates parkinsonian deficits via dual modulation of neuroinflammation and gut microbiota in MPTP-induced models.

    Brain research·2026
    Same journal

    Emotions, the amygdala, and the right hemisphere.

    Brain research·2026
    See all related articles

    Primary rat or mouse brain cultures primarily contain glial cells, including macrophages and endothelial cells. Few differentiated neurons were detected, indicating these cultures are suitable for studying glial cell functions.

    Area of Science:

    • Neuroscience
    • Cell Biology

    Background:

    • Primary brain cultures are crucial for studying neural cell types.
    • Characterizing cell populations in these cultures is essential for accurate experimental interpretation.

    Purpose of the Study:

    • To identify and characterize the major cell types present in primary cultures of rat or mouse brain hemispheres.
    • To assess the suitability of these cultures for studying glial cell biology.

    Main Methods:

    • Cell culture from brain hemispheres.
    • Immunofluorescence staining using specific antisera (anti-S-100, antimacrophage, anti-14-3-2).
    • Assessment of phagocytotic activity and alkaline phosphatase activity.

    Main Results:

    • Cultures predominantly consisted of glial cells, identified by [3H]GABA uptake and S-100 antiserum reactivity.

    Related Experiment Videos

  • Mesodermal macrophages were identified by antimacrophage antiserum and phagocytosis.
  • Endothelial cells were identified by alkaline phosphatase activity, increasing with culture age.
  • Differentiated neurons were largely absent, as indicated by negative anti-14-3-2 antiserum reactions.

    • Conclusions:

    • Primary brain hemisphere cultures are rich in glial cells, including macrophages and endothelial cells.
    • These cultures contain minimal differentiated neurons, making them ideal for glial cell research.