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[Microsomal epoxide hydrolase (author's transl)]

P M Dansette

    Annales De Biologie Clinique
    |January 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Microsomal epoxide hydrolase detoxifies harmful epoxides into excretable compounds. This enzyme, crucial in metabolism, can also activate toxic substances like polycyclic hydrocarbons.

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    Area of Science:

    • Biochemistry
    • Enzymology

    Context:

    • Microsomal epoxide hydrolase (mEH) catalyzes epoxide hydration.
    • mEH exhibits broad specificity for certain epoxides but is inactive on others.
    • The enzyme is stereospecific, yielding (R,R)-dihydrodiols.

    Purpose:

    • To characterize the properties and function of microsomal epoxide hydrolase.
    • To investigate the enzyme's role in epoxide metabolism and detoxification.
    • To understand the enzyme's involvement in the bioactivation of xenobiotics.

    Summary:

    • Microsomal epoxide hydrolase (mEH) is a 49,000 MW hydrophobic protein found in rat and human liver microsomes.
    • It hydrates epoxides to hydrophilic dihydrodiols, aiding excretion.
    • Despite high concentrations, its membrane-bound nature complicates kinetic analysis.

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    Impact:

    • mEH plays a dual role: protecting against reactive epoxides and potentially activating toxic compounds.
    • Understanding mEH is vital for xenobiotic metabolism and toxicology, particularly concerning polycyclic aromatic hydrocarbons.