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Transcription termination factor rho and T-even phage development

Y N Zograff, A L Gintsburg

    Molecular & General Genetics : MGG
    |January 1, 1980
    PubMed
    Summary

    Functional factor rho is essential for T-even phage development, impacting protein synthesis and DNA replication. Mutations affecting rho significantly disrupt phage processes, highlighting its critical role.

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Virology

    Background:

    • T-even phages (T2, T4) require functional factor rho for development.
    • Rho activity levels influence phage development, with different phages needing varying degrees of activity.
    • Inactivation of rho in E. coli leads to significant disruptions in phage protein synthesis and DNA replication.

    Purpose of the Study:

    • To investigate the essential role of functional factor rho in T-even phage development.
    • To elucidate the specific mechanisms by which rho influences phage protein synthesis and DNA replication.
    • To explore the relationship between rho activity and bacteriophage DNA replication.

    Main Methods:

    • Utilizing temperature-sensitive (ts) mutations in E. coli to inactivate functional factor rho.
    • Analyzing the effects of rho inactivation on early and late protein synthesis during phage infection.
    • Investigating the impact of rho mutations on phage DNA replication and capsid protein maturation.
    • Isolating and characterizing rifampicin-resistant mutations that suppress rho mutations.

    Main Results:

    • Rho inactivation reduces early protein synthesis and causes premature formation of later-stage proteins.
    • Capsid protein maturation is impaired, and phage DNA replication is sharply inhibited in rho-inactivated cells.
    • All proteins necessary for phage DNA synthesis are formed without functional rho, indicating its direct involvement in replication.
    • RNA polymerase mutations partially or completely fail to suppress rho mutation effects on phage development and DNA synthesis.

    Conclusions:

    • Functional factor rho is indispensable for T-even phage development, particularly for DNA replication.
    • Rho's role extends beyond transcription, directly influencing the replication process of T-even phage DNA.
    • While RNA polymerase is involved, it does not fully compensate for the loss of rho function in phage development.

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