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Enzymic micro-assay for blood glutamine

A Stahl, A Frick, M Imler

    Clinical Chemistry
    |October 1, 1978
    PubMed
    Summary

    This study presents a new microassay for measuring blood glutamine levels using a continuous-flow colorimetric method. The assay is sensitive and accurate for determining glutamine concentrations in human and rat blood samples.

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    Area of Science:

    • Biochemistry
    • Clinical Chemistry
    • Analytical Chemistry

    Background:

    • Glutamine is a crucial amino acid involved in various metabolic pathways.
    • Accurate measurement of blood glutamine is essential for understanding metabolic states and diseases.

    Purpose of the Study:

    • To develop and optimize a sensitive microassay for quantifying blood glutamine concentrations.
    • To establish reference ranges for blood glutamine in humans and rats using the developed assay.

    Main Methods:

    • Hydrolysis of blood glutamine by glutaminase.
    • Dialysis of released ammonia.
    • Measurement of ammonia using a continuous-flow colorimetric microassay.
    • Optimization of assay conditions for micro-scale analysis.

    Main Results:

    • The microassay can accurately determine glutamine concentrations from 10 to 1300 micromol/liter in a 40-microliter sample.
    • Higher concentrations up to 5 mmol/liter can be measured with minor modifications.
    • Reference ranges established: Human arterial blood (443 ± 140 µmol/L), human venous blood (466 ± 142 µmol/L), rat arterial blood (320 ± 92 µmol/L), rat venous blood (340 ± 108 µmol/L).
    • Fasting was observed to decrease blood glutamine levels.

    Conclusions:

    • A reliable and efficient continuous-flow colorimetric microassay for blood glutamine measurement has been developed.
    • The assay provides accurate and sensitive quantification of glutamine in small sample volumes.
    • Established reference values offer a baseline for further clinical and research applications, highlighting the impact of fasting on glutamine levels.

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